KMID : 0364820070430010066
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Korean Journal of Microbiology 2007 Volume.43 No. 1 p.66 ~ p.71
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Lee Ha-Yan
Lee Sang-Seob Yoo Yong-Kyu Seo Pil-Soo Lee Jung-Sook Lee Keun-Chul
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Abstract
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Two isolates of genus Acinetobacter were obtained from Jang-Baek waterfall in North Korea. Morphological characteristics of the isolated 2 strains were Gram-negative, aerobic and rod shape bacteria. Physiological and biochemical characterization of the isolated 2 strains were some different aspect from those of type strains.
16S rDNA sequence analysis showed that the two isolates shared 99.9% sequence similarity. Strains JB10 and JB15T were shown to belong to the Gammaproteobacteria and showed the highest levels of sequence similarity to Acinetobacter tandoii 4N13T (97.3%), Acinetobacter haemolyticus ATCC17906T (97.2%), Acinetobacter johnsonii DSM6963T (97.2%), Acinetobacter junii DSM6964T (96.7%), Acinetobacter schindleri LUH5832T (97.0%) and Acinetobacter ursingii LUH3792T (96.6%). The major cellular fatty acid in Acinetobacter type strains and isolated strains included C18:1 ¥ø9c and C16:1 ¥ø7c/C15:0 iso 2OH. Eventhough it was ascertained that the isolated strains were closely related to genus Acinetobacter, physiological and biochemical characteristics and the result of the isolated strains 16S rDNA analysis indicate some different aspects from those of type strains of genus Acinetobacter. It is considered that the isolated JB10 (= KEMC 52-093) and JB15T (KEMC 52-094T) strains be new species of genus Acinetobacter. We name it as Acinetobacter koreensis sp. nov.
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KEYWORD
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16S rDNA sequence, Acinetobacter koreensis, fatty acid
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